discrimination and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer sff-sfr
نویسندگان
چکیده
self-incompatibility in almond and prunus species is an important genetic trait that prevents self-fertilization. self-incompatibility in almond is controlled gametophytically by the multiallelic s-locus. this study was done in order to identification and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer sff-sfr.some important morphological traits of parental crosses were evaluated using almond descriptor. also, offspring’s (f1) of five crosses including; a (tuono × 101 genotype), b (supernova × 101 genotype), c( genco × shahrood 21), d (tuono × shahrood 12) and e (tuono × shahrood 17) were tested using pcr in order to dna amplification and self-compatibility evaluation. results of pcr method found that self-compatible progenies showed 449 bp band, while this band not observed in self-incompatible progenies. in addition, all self-incompatible progenies appeared no s1 allele at any condition.
منابع مشابه
Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer
Self-incompatibility in almond and Prunus species is an important trait that prevents self-fertilization. Selfincompatibility in almond is controlled gametophytically by the multiallelic S-locus. Present study was done in order to identification and preliminary selection of self compatible progenies resulted from controlled crosses in almond using specific primer SfF/SfR. Some important morphol...
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عنوان ژورنال:
به نژادی گیاهان زراعی و باغیجلد ۱، شماره ۲، صفحات ۱۱۱-۱۲۲
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